Lyssavirus Detection and Typing Using Pyrosequencing 1
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Lyssavirus detection and typing using pyrosequencing.
Rabies is a fatal zoonosis caused by a nonsegmented negative-strand RNA virus, namely, rabies virus (RABV). Apart from RABV, at least 10 additional species are known as rabies-related lyssaviruses (RRVs), and some of them are responsible for occasional spillovers into humans. More lyssaviruses have also been detected recently in different bat ecosystems, thanks to the application of molecular d...
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Significant advances in the techniques available for rabies diagnosis have taken place over the past thirty years. The traditional method of histological examination for Negri bodies in the brain sections has been largely replaced by immunofluorescent staining methods for the detection of nucleocapsid antigen in brain smears (fluorescent antibody test: FAT) (Dean and Abelseth, 1973). Neverthele...
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The ability to determine nucleic acid sequences is one of the most important platforms for the detailed study of biological systems. Pyrosequencing technology is a relatively novel DNA sequencing technique with multifaceted unique characteristics, adjustable to different strategies, formats and instrumentations. The aims of this thesis were to improve the chemistry of the Pyrosequencing techniq...
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BACKGROUND Pyrosequencing can be applied for Single-Nucleotide-Polymorphism (SNP)-based pathogen typing or for providing sequence information of short DNA stretches. However, for some pathogens molecular typing cannot be performed relying on a single SNP or short sequence stretch, necessitating the consideration of several genomic regions. A promising rapid approach is the simultaneous applicat...
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تاریخ انتشار 2011